Researchers from Brigham and Women’s Hospital and the Massachusetts Institute of Technology (MIT) have unveiled previously unseen details in human brain tissue through a new, cost-effective, microscopy technology.
The key points are:
- Researchers have developed a new microscopy technology called „Decrowding Expansion Pathology“ (dExPath) to analyze brain tissue.
- By disassembling proteins with dExPath, researchers can stain proteins in tissues that were previously inaccessible, highlighting nanometer-sized structures or even cell populations that were previously hidden.
- This „Super-Resolution Imaging“ technology has the potential to provide insights that could improve diagnostic strategies and patient outcomes.
This new microscopy technology called „Decrowding Expansion Pathology“ (dExPath) developed by researchers at Brigham and Women’s Hospital and MIT, has revealed unprecedented details in brain tumor tissue, results published in Scientific Translational Medicine provide new insights into the development of brain tumors with potential implications for the advancement of the diagnosis and treatment of aggressive neurological diseases.
„In the past, we have relied on expensive high-resolution microscopes that only well-equipped labs could afford, required specialized training to use, and were often impractical for high-throughput analyses of brain tissue at the molecular level,“ said Pablo Valdes, MD, PhD, a graduate of neurosurgery at Brigham and lead author of the study. „This technology brings reliable, high-resolution imaging into the clinic, enabling scientists to study neurological diseases at an unprecedented nanoscale in conventional clinical samples using conventional microscopes.“
Earlier, researchers relied on costly, super high-resolution microscopes to image nanoscale structures in cells and brain tissue and, even with the most advanced technology, often had difficulties effectively capturing these structures at a nanoscale level.